Mexico City. December 9, 2024. During the 14th Annual Conference of the Mexican College of Cellular Therapy and Regenerative Medicine, the preliminary results of a basic clinical research project carried out by doctors Dr. Donato Pérez García and Dr. Alejandra Calderón, Hematologist who was in charge of carrying out all the measurements in the blood sample of each of the ten patients who participated in this study. The results are very good and add evidence of how the effect of insulin is important to enhance and generate changes at the genetic level in the cell, in addition to the fact that since it is a natural hormone in our body, the entire treatment technique uses normal mechanisms. or physiological, of the organism.
This point is important because the natural mechanisms of cellular functioning are used with a substance that the body uses daily and does not require the creation and use of synthetic stimulators that would not be completely tolerated by the cell. The anecdotal evidence of more than 90 years of the use of this technique in medical therapy already has another positive result.
Below is a summary of this research study. Below is a summary of this research study.
BASIC INVESTIGATION.
NON-DIABETIC USE OF INSULIN IN THE TREATMENT OF SOME NEOPLASMS USING THE INSULIN POTENTIATION TECHNIQUE.
Evaluation of the expression of the tumor suppressor protein p53 in cancer patients receiving IPT therapy
In 1931, the German researcher Otto Warburg observed that cancer cells metabolized glucose differently, because their mitochondria were altered, thus directing the metabolism towards glycolysis, for this discovery he received the Nobel Prize.
Increase in TNF, IL-1β, IL-6 and IL-8 (pro-inflammatory cytokines).
Production of ROS and lipid peroxidation (favors the control of oxidative stress).
Theories all based on cellular metabolism and not related to alterations at the genetic level.
p53 at rest its expression is decreased, and it increases in the presence of cellular stress.
The prevalence of p53 mutations varies significantly by cancer type and also depends on the stage of tumor development, with prevalence’s ranging from less than 5% in cervical cancer and 10% in leukemia to 80% in non-small cell lung cancer and 90% in ovarian cancer.
C-myc. It is located in cr.8, under normal conditions they are regulated.
Amplification of the c-Myc gene is very common in breast, lung, ovarian, prostate cancer, leukemias and lymphomas; while loss of regulation is more common in colon cancer, gynecological tumors and melanoma.
Objective: Demonstrate how p53 is expressed when the tumor cell is subjected to hypoglycemia as a mechanism of cellular stress.
Methodology: 10 patients who attended IPT therapy with Dr. Donato were included.
After obtaining informed consent, in a hospital environment and with hemodynamic monitoring.
CONCLUSIONS
Hypoglycemia turns out to be a stimulus for the expression or suppression of genes that participate in the cell cycle.
It was observed how tumor cells, regardless of the tissue in which they develop, present a decrease in tumor suppressor genes and an increase in oncogenes.
More studies are needed to corroborate whether the longer the duration of hypoglycemia, the expression of p53 is favored and a decrease in the percentage of tumor cells that express c-myc is observed.
Confirm if the level of hypoglycemia has a direct relationship with the percentage of cells that express tumor suppressor genes.
With these findings it can be suggested that IPT therapy has an epigenetic basis that influences genetic expression or suppression at the cellular level.
Dr.Donato Perez Garcia
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